Plasmid

Part:BBa_K3179105

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)


pT2mKE

pT2mKE(pTRE-mi663b-2Kt-E1A-miR885-5p)


Usage and Biology

This part consists of miR663b-target, 2kt-E1A and miR885-5p-target,which be put in the vector pTRE. From promoter CMV our plasmid can transcribe mRNA with miR663b-target, 2kt-E1A and miR885-5p-target, whose translation can be regulated by level of free miRNA663b and miRNA885-5p, and also by L7Ae. When they exist, the translation of this mRNA will be suppressed. The production is E1A, which can start the expression of adenoviral gene. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 999
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 999
    Illegal NgoMIV site found at 473
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None